The 4 day workshop on Cryo-sectioning and Immuno- Electron Microscopy (EM) is meant for people willing to learn cryo-sectioning and Tokuyasu Technique for immune-EM. Experience in resin sectioning is not necessary, but is definitely an advantage. This workshop is hands-on in nature, although some topics will be also covered with lectures. The entire EM procedure from (live) specimen to imaging immuno-labeled ultrathin sections will be covered from start to finish. You will have the chance to use most of the workshop time in the lab working with standard specimens like cultured cells or tissues. Besides managing the ultra-cryo-microtome, the preparation of EM tools and other necessities will be covered.
We provide an excellent learning environment. When manual skills like ultrathin sectioning of resin blocks are being trained the participants will have their own ultra-cryo-microtome (often equipped with a camera) to practice on. The number of participants is limited to 12 people. We will provide you with a practical manual and detailed protocols covering the topics of the workshop.
For the preliminary program and a more elaborate list of topics please visit: https://www.cellbiology-utrecht.nl/electron-microscopy-courses/cryosectioning-and-immuno-electron-microscopy.html