Ph.D / Postdoc course Advanced Optical Microscopy 2020

June 15 – 19, 2020

Aim: The aim of this one-week course is to introduce, discuss, and experience the technologies and applications of various advanced optical microscopic techniques. Also, tissue and cell preparation protocols and immune-cytochemical procedures will be discussed. Finally, vital imaging and quantification of molecular processes in the cell will be explained and demonstrated.

Target group: PhD and Postdoc students in the Life Sciences.

Content: This course is composed of lectures, practical training, and demonstrations on the following topics:

  • Basic microscopy – Fluorescence (spectroscopic) microscopy – Confocal laser scanning laser microscopy
  • Light sheet microscopy – High-throughput microscopy
  • Super-resolution microscopy (with a focus on STED)
  • Multiphoton laser scanning laser microscopy
  • Vital imaging of cells and tissues
  • Fluorescence resonance energy transfer (FRET)
  • Fluorescence lifetime imaging (FLIM)
  • Sample preparation and clearing
  • 3D image restoration and image processing

Approach: Theoretical lectures will be provided in the morning sessions, while practical training and demonstrations are planned in the afternoon. You will have the opportunity to discuss your results or view your own microscopic preparations with experts in the field. The course will end with a one day symposium, the annual Maastricht Microscopy Meeting (MMM) on Advanced Optical Microscopy.

Examination: At the start of the course students will receive by email an open question, dealing with a cell biological problem that should be solved by using microscopic techniques and proper preparation of the sample. Students may do this assignment individually or in small groups. The deadline for the assignment is Friday June 26th 2020. Examination results will be available by July 24th 2020 at the latest.

Min/Max students: maximum 20 participants

Location: Faculty of Health, Medicine and Life Sciences, Maastricht University Medical Center

Coordinator: Prof.dr. Marc van Zandvoort, Dept. of Molecular Cell Biology, Maastricht University Medical Center, PO Box 616, 6200 MD Maastricht, tel: +31 43 3881361,

Teaching staff: M. van Zandvoort, J. Broers, D. Kapsokalyvas, T. Rademakers, and others (to be announced).

Registration: Secretary Molecular Cell Biology / / +31 43 3881351

Registration fee: € 200

Basics in Fluorescence Microscopy – Amsterdam, NL

Dear colleagues,

We are happy to announce the course “Basics in fluorescence microscopy”, organised by the van Leeuwenhoek Centre for Advanced Microscopy at Amsterdam University, the Netherlands, January 28th-31st, 2020.

This is a 4-days basic microscopy course for graduate students, postdocs and lab technicians in biology, biophysics and (bio)medicine. It provides detailed knowledge of the working principles of confocal and widefield fluorescence imaging, with special emphasis on experiment related issues, such as optical aberrations, bleaching, specimen preparation and fluorescent probes. The course integrates theoretical lectures with hands-on experiments and practical experience. Experts in the field of microscopy will give an overview of “state-of-the-art” imaging techniques in biological research.

For more info, program and application form check

Participants: Max. 20 PhD students, postdocs and technicians.
Date: January 28th-31st 2020
Level: Starting microscopist
Costs (including coffee and lunches):  400 €
Application deadline: January 3rd 2020
Organisers: LCAM, Amsterdam, the Netherlands, contact: Mark Hink

NeCEN EM Seminar – Dr. Rouslan Efremov

Dear EM enthusiasts,

The next presentation from the NeCEN seminar series on Electron Microscopy will be on Wednesday December 11 (15:30) in room GM 4.13 at the Gorlaeus Laboratories Betacampus. Dr. Rouslan Efremov from the VIB-VUB in Belgium will present.

Dr. Efremov has setup a cryoEM facility for single particle work with a JEOL cryoARM300; one of the two in Europe. He will give us an update on the performance of the machine and his own research interests which lie on the structural biology of large protein complexes using single particle as the main technique.

If you would like to receive announcements about NeCEN events, please send an email to and we will add your name to our mailing list.

Looking forward to seeing you there,
The NeCEN team