The Dutch Cryo-EM School will take place from 27 June to 02 July 2022 in Leiden and Delft. The aim of this school is to teach the basic principles and practical aspects of image processing to structural and molecular biologists wishing to determine macromolecular structures by cryoEM. The course will cover all aspects of cryoEM structure determination, ranging from sample preparation over data collection to processing and interpretation of high-resolution image data, for both single particle images and tomograms, and will be aimed at advanced PhD students and postdocs using cryoEM for structural analysis.

Application and registration:

The event is limited up to 20 participants. For selection purposes, please note that your application will not be considered without motivation.

During registration, please provide a brief motivation (max. 300 words) including a short description of your project, your level of prior experience with cryo-EM/ET and how you and your project will benefit from this school. You will also need to provide the name of your PI. The results will be announced approximately 2-3 weeks after the application deadline on 02 May 2022.

Registration fees:

There are no registration fees for participants from academia. The program includes lunch/coffee on all course days, and the course dinner on Wednesday 29 June. Transportation to and from the venues, and accommodation, are not included in the school and need to be arranged by the participants.

The 4 day workshop on Cryo-sectioning and Immuno- Electron Microscopy (EM) is meant for people willing to learn cryo-sectioning and Tokuyasu Technique for immune-EM. Experience in resin sectioning is not necessary, but is definitely an advantage. This workshop is hands-on in nature, although some topics will be also covered with lectures. The entire EM procedure from (live) specimen to imaging immuno-labeled ultrathin sections will be covered from start to finish. You will have the chance to use most of the workshop time in the lab working with standard specimens like cultured cells or tissues. Besides managing the ultra-cryo-microtome, the preparation of EM tools and other necessities will be covered.

We provide an excellent learning environment. When manual skills like ultrathin sectioning of resin blocks are being trained the participants will have their own ultra-cryo-microtome (often equipped with a camera) to practice on. The number of participants is limited to 12 people. We will provide you with a practical manual and detailed protocols covering the topics of the workshop.

For the preliminary program and a more elaborate list of topics please visit: https://www.cellbiology-utrecht.nl/electron-microscopy-courses/cryosectioning-and-immuno-electron-microscopy.html

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The 3 day workshop on resin electron microscopy (EM) is suitable for people that do not have previous experience with standard electron microscopy sample preparation techniques. This workshop is primarily hand-on in nature, although some topics will be covered with lectures. The entire EM procedure from (live) specimen to imaging stained ultrathin resin sections will be covered from start to finish. You will have the chance to use most of the workshop time in the lab working with standard specimens like cultured cells or tissues. Besides embedding in a resin and managing the ultra-microtome, the preparation of EM tools and other necessities will be covered.

We provide an excellent learning environment. When manual skills like ultrathin sectioning of resin blocks are being trained the participants will have their own ultra-microtome (often equipped with a camera) to practice on. The number of participants is limited to 12 people. We will provide you with a practical manual and detailed protocols covering the topics of the workshop.

For the preliminary program and a more elaborate list of topics please visit: https://www.cellbiology-utrecht.nl/electron-microscopy-courses/resin-electron-microscopy.html