Webinar: Super-resolution techniques and focus on nuclear pore complex

An important metric for assessing a system’s utility for visualizing structures is the microscope’s resolving power. As technologies emerge, particularly in super-resolution fluorescence microscopy, there is a need for quantifying the resolution. While there are several strategies using test patterns for such a measurement, there is also a need for biologically-relevant control samples that can serve as a benchmark. In this talk, we discuss assessing image resolution, particularly the use of the arrangement of proteins in the nuclear pore complex to characterize resolution in super-resolution microscopes.

More info here

NEMI day 2023: Photo impression

On behalf of TU Eindhoven and NEMI we would like to thank all speakers, sponsors and participants of the 2023 NEMI Day Thanks to you the day was a great succes! 

Please find below a photo impression of the day. Photography by Luuk Moone.

The 2023 NEMI Day is sponsored by:

SVI Analyzing objects within other objects and object organization, using clusters and convex hull operations

If you image analysis questions concern the organization or properties of objects, or the grouping of objects within other objects such as for example nuclei or cells, then this webinar may interest you. We will show you how tho use the latest features in the Huygens Object Analyzer to not only analyze object in detail, but also to count and analyze the properties of objects within objects (cells, nucleus, organelles) in the other channel. The organization of objects can also be determined within just a single channel by clustering them using the Cluster Analyzer. And, geometric analysis of clusters is facilitated with the newly implemented convex hull option, which basically works like placing a rubber balloon around a cluster of objects. Convex hull based analysis also can aid the analysis of non-homogeneously stained objects, as is often teh case with for example DAPI-stained nuclei. The ‘rubber balloon’/convex hull of the object then helps to fill the holes in the segmentation for the non-homogeneous staining. Register here for this webinar.

October 12 NEMI day 2023

Dear NVvM community,

Registration for the 2023 NEMI Day is now open

We are delighted to invite you to the next NEMI Day, dedicated to collaboration and knowledge exchange among the Dutch Electron Microscopy community. This year the NEMI day will take place at the Eindhoven University of Technology on October 12 and the theme is ‘imaging processes’.  The NEMI day will encompass keynote speakers, contributed talks, a poster session and networking opportunities. 

Prior to the meeting (9:00-10:30) we organized a site visit to ThermoFisher Scientific. We encourage particularly early-career scientists to attend! 

Register here

SVI Analysis pipelines & Batch Processing; Using Huygens Command Line Interface

Huygens Core can be configured for maximum performance offered by computer nodes, large scale 64 bit multiprocessors, and systems with multiple (high-end) Nvidia GPU cards. The Huygens Core Compute Engine can be called from any pipeline environment using our new Command Line Interface (CLI). Run all of Huygens’ sophisticated image processing tasks, as available in the Workflow Processor, with a node/job managment software such as SLURM (Trademark of SCHEDMD LLC), or from an analysis pipeline scripted with for example Jupyter (Trademark of NumFOCUS foundation). If you do not want to program yourself and are interested in using the Core for batch image processing or with many users, you can use the customizable front-end Huygens Remote Manager. This open source task manager acts as a web-interface to Huygens Core that can be can be used with any web-browser. Aside from these topics, we address during this webinar the new tasks added to the Workflow Processor, such as crosstalk correction and the 3D Object AnalyzerRegister here for this webinar.