Join us for the next NEMI platform meeting in which Marijn van Huis give a colloquium.

Date: April 19th
Registration: link

Abstract

Advances in Liquid Phase Electron Microscopy: Imaging Strategies for Soft Materials and Controlled Motion of Nanoparticles 

In-situ cells developed for the transmission electron microscope (TEM) allow performing experiments on nanoscale materials in liquids and gases, even while heating or under application of electric fields, while imaging them at near-atomic resolution. Of these two, in particular liquid cell TEM (LC-TEM) imaging is particularly sensitive to e-beam effects [1]. In this seminar I will discuss recent progress made at Utrecht University in the imaging of induced transitions. The first model system is p-NIPAM, a thermoresponsive biocompatible polymer that reversibly compresses when heated from room temperature to a temperature of 40 °C and vice versa. When immersed in water and thus swollen, the density of p-NIPAM is almost the same as that of water, while it is extremely sensitive to e-beam irradiation. Eventually a suitable approach was found and the thermoresponsive response was successfully imaged [2]. The second model system is a ‘rattle particle’ consisting of a mobile core particle inside a larger silica shell where the porous shells are filled with liquid. Depending on the salt concentration in the liquid, the potentials between the core and shell could be adapted to confine the core particle at the center of the shell, or be adapted in such a way that the core particle could explore nearly the entire volume of the spherical cavity [3]. Finally, preliminary results on the application of an AC electric field on the rattles show that the direction of the motion of the cores, either parallel or orthogonal to the field, could be controlled depending on the frequency of the applied field. 

[1] T.A.J. Welling, S. Sadighikia, K. Watanabe, A. Grau-Carbonell, M. Bransen, D. Nagao, A. van Blaaderen, M.A. van Huis, ‘Observation of undamped 3D Brownian motion of nanoparticles using liquid-cell scanning transmission electron microscopy’, Part. Part. Syst. Charact. 37 (2020), 202000003, pp 1-10. DOI: 10.1002/ppsc.202000003. 

[2] A. Grau-Carbonell, F. Hagemans, M. Bransen, N. Elbers, R. J. A. van Dijk-Moes, S. Sadighikia, T. A. J. Welling, A. van Blaaderen, M. A. van Huis, ‘Direct in-situ characterization of the thermoresponsive and cononsolvent behavior of Poly(N-isopropylacrylamide) microgels and shells with nanometer resolution’, in preparation. 

[3] T.A.J. Welling, K. Watanabe, A. Grau-Carbonell, J. de Graaf, D. Nagao, A. Imhof, M.A. van Huis, A. van Blaaderen, ‘Tunability of Interactions between the Core and Shell in Rattle-Type Particles Studied with Liquid-Cell Electron Microscopy’, ACS Nano 15 (2021) 11137-11149. DOI: 0.1021/acsnano.1c03140

FOCUS ON MICROSCOPY 2022
April 10 – 13, 2022
Porto, Portugal
Incorporating:
35th International Conference on 3D Image Processing in Microscopy 
34th International Conference on Confocal Microscopy

Dear Colleagues,

 Abstracts for oral and poster presentations can now be submitted through the conference website: www.FocusOnMicroscopy.org.

 Deadline for abstract submission: Tuesday, January 25, 2022.

After the successful FOM2021 online conference earlier this year, it is a pleasure to announce FOM2022: the next in a series of unique interdisciplinary meetings on advanced and multi-dimensional light microscopy and image processing. We are optimistic that the COVID-19 situation will again allow an in-person meeting. The FOM2022 conference takes place in the historic city centre of Porto, by the Douro River at the 

Centro de Congressos da Alfândega do Porto (www.ccalfandegaporto.com/en/).

The Porto conference will start on Sunday morning April 10 with tutorials, followed by parallel sessions, Flash poster presentations and a plenary opening session with invited speakers at the end of the afternoon. The first day will be closed with a welcome reception.

If you wish to be kept informed on updates, please leave your email address here. A more detailed announcement will be sent early next year. 

FOM2022 continues a long-standing (since 1988), yearly conference series on the latest innovations and developments in (optical) microscopy and their application in biology, medicine, and the material sciences. FOM is committed to advancing microscopy research and meeting the needs of participants by providing opportunities for sharing work and connecting scientists with the global scientific community.

Key topics include:

• Theory and practice of confocal and multiphoton-excitation microscopy
• Super-resolution, nanoscopy imaging: from PSF engineering (4pi, SIM, STED), fluorescent activation/quenching, stochastic/centroid (PALM, STORM, GSDIM, SOFI and related techniques) to TIRF
• 3D and 4D live cell and tissue imaging
• Adaptive optics for microscopy
• Light sheet microscopy
• Phase/interference based microscopies
• OCT, holographic, endoscopy
• Advanced fluorescence imaging/spectroscopy: FRET, FRAP, FLIM, FCS, SOFI
• New fluorescence probes, proteins, quantum dots, single molecule imaging
• Clearing and expansion techniques.
• Coherent non-linear microscopies: SHG, THG, SFG, CARS.
• Multi-dimensional fluorescence and Raman spectroscopy imaging
• Correlated light/electron microscopy
• Laser manipulation and tracking, photo-activation
• OCT, endoscopy
• Fast acquisition, automated and high-content microscopy
• 3D image processing and visualization for multidimensional data

 
Abstracts for contributions are invited and can be submitted starting Sunday October 24 through our website: www.FocusOnMicroscopy.org where further information on the present and previous FOM conferences can be found.

Important dates:

Deadline for the submission of abstracts	Tuesday, January 25, 2022
Acceptance of abstracts, program on the web	Thursday, February 17, 2022
Deadline for early registration	Thursday, March 10, 2022
FOM2022 Conference	Sunday, April 10 – April 13, 2022
Easter Sunday 2022	April 17, 2022

Welcoming you to the Porto FOM2022 conference and exhibition,
the FOM2022 organizers:

• Paula Sampaio, IBMC/i3S, University of Porto, Portugal
• Hans Gerritsen, Utrecht University, The Netherlands
• Fred Brakenhoff, University of Amsterdam, The Netherlands